The ImmunoSpot IFN-γ/TNF-α/IL-6 assay is the first ever triple color mouse FluoroSpot. Detect cells secreting each cytokine individually or in combination(s) with accuracy comparable to single-color (single cytokine) analysis. Stimulated effector cells can be detected

The AlphaLISA Mouse TNFα Biotin Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum cell culture medium and other samples types using a homogeneous no wash steps no separation steps

This optimized FluoroSpot assay reliably detects and measures mouse TNF-α and IL-4 secretions by stimulated effector cells in frequencies as low as one in a million. Scanning and analysis requires an analyzer with fluorescent capabilities.

An ELISA kit for the detection of TNFα Mouse This uses Sandwich ELISA Double Antibody and has a sensitivity of 18 75pg ml

Intracellular staining of TNF-α–producing mouse cells

This optimized ELISPOT assay reliably detects and measures mouse TNF-α and IL-2 secretions by stimulated effector cells in frequencies as low as one in a million.

This standard should be reconstituted in 100 µL Milli Q grade water The reconstituted analyte should be used within 60 minutes or aliquoted into screw capped polypropylene vials and stored at 20°C for further experiments

Intracellular staining of TNF-α–producing mouse cells